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Primary structure of α-globin chains from river buffalo (Bubalus bubalis L.) hemoglobins

Ferranti, Pasquale and Facchiano, Angelo and Zappacosta, Francesca and Vincenti, Donatella and Rullo, Rosario and Masala, Bruno Lucio and Di Luccia, Aldo (2001) Primary structure of α-globin chains from river buffalo (Bubalus bubalis L.) hemoglobins. Journal of Protein Chemistry, Vol. 20 (2), p. 171-179. eISSN 1573-4943. Article.

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DOI: 10.1023/A:1011027924391


Primary structure analysis of the four river buffalo α-globin chains showed that haplotypes A and B differ from each other by a substitution at codon 64 that may encode Ala or Asn. The A haplotype encodes two α -globin chains, I α1 and II α3, which differ at positions 129 and 131: I α1 has 64 Ala, 129 Phe, 131 Asn; II α3 has 64 Ala, 129 Leu, 131 Ser. The B haplotype encodes two α-globin chains, I α2 and II α4, which differ at positions 10 and 11: I α2 has 10 Ile, 11 Gln, 64 Asn; II α4 has 10 Val, 11 Lys, 64 Asn. Apart from the Ala/Asn polymorphism at position 64, amino acid substitutions in allelic and nonallelic α-globin chains seem to have arisen by single point mutations. Detection of electrophoretically silent mutations due to neutral amino acid substitutions and their influence on the isoelectric point are discussed. Furthermore, primary structures of river buffalo α-globin chains are compared to other species of the Bovidae family to suggest evolutionary events that have characterized the amino acid substitutions of river buffalo hemoglobin.

Item Type:Article
ID Code:3064
Uncontrolled Keywords:α-Globin chain, primary structure, river buffalo hemoglobin, molecular modeling, mass spectrometry
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Springer Netherlands
Deposited On:29 Sep 2009 09:53

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