Pirastru, Monica and Palici di Suni, Marcella and Vacca, Giuseppe Massimo and Franceschi, Paul and Masala, Bruno Lucio and Manca, Laura (2000) The Hemoglobin polymorphism in Sardinian goats: nucleotide sequence and frequency of βA, βD, βD-Malta, and βE globin genes. In: Di Prisco, Guido and Giardina, Bruno and Weber, Roy Edwin (eds). Hemoglobin function in vertebrates: molecular adaptation in extreme and temperate environments, Milano - New York, Springer. p. 97-108. ISBN 88-470-0107-7. Book Section.
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A study was conducted in hundreds of goats living in Sardinia by means of isoelectric focusing (IEF) of Hb tetramers and electrophoresis of dissociated globin chains. This screening showed that Sardinian breeds are also characterized by a notably high Hb polymorphism. Here, we report the results of this screening and our findings on nucleotide sequence analysis of the four most common allelic β-globin genes, all characterized by distinct electrophoretic patterns. These are the βA, βD, βD-Malta, and βE genes. It is expected that with the methodologies described here the various α and β chain variants present in goat can be completely characterized.
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