Tombelli, Sara and Mascini, Marco and Scherm, Barbara and Battacone, Gianni and Migheli, Quirico (2009) DNA biosensors for the detection of aflatoxin producing Aspergillus flavus and A. parasiticus. Monatshefte für Chemie = Chemical Monthly, Vol. 140 (8), p. 901-907. eISSN 1434-4475. Article.
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In this work, we report on the development of a DNA-based piezoelectric biosensor specific for the detection of an amplicon of the aflD gene of Aspergillus flavus and A. parasiticus. Expression of this gene is consistently correlated with a strain’s ability to produce aflatoxins that are considered very potent liver carcinogens in various animal species and humans. The DNA biosensor has been characterized with synthetic oligonucleotides and amplicons. Moreover, it has been applied to the analysis of real samples consisting of amplicons of DNA extracted from flours and feed contaminated with A. flavus and A. parasiticus.
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