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DNA biosensors for the detection of aflatoxin producing Aspergillus flavus and A. parasiticus

Tombelli, Sara and Mascini, Marco and Scherm, Barbara and Battacone, Gianni and Migheli, Quirico (2009) DNA biosensors for the detection of aflatoxin producing Aspergillus flavus and A. parasiticus. Monatshefte für Chemie = Chemical Monthly, Vol. 140 (8), p. 901-907. eISSN 1434-4475. Article.

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DOI: 10.1007/s00706-009-0137-3

Abstract

In this work, we report on the development of a DNA-based piezoelectric biosensor specific for the detection of an amplicon of the aflD gene of Aspergillus flavus and A. parasiticus. Expression of this gene is consistently correlated with a strain’s ability to produce aflatoxins that are considered very potent liver carcinogens in various animal species and humans. The DNA biosensor has been characterized with synthetic oligonucleotides and amplicons. Moreover, it has been applied to the analysis of real samples consisting of amplicons of DNA extracted from flours and feed contaminated with A. flavus and A. parasiticus.

Item Type:Article
ID Code:3053
Status:Published
Refereed:Yes
Uncontrolled Keywords:Biosensors, DNA, polymerase chain reaction, food, Aspergillus
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Springer Wien
eISSN:1434-4475
Deposited On:25 Sep 2009 10:22

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