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Bacterial flora associated with the digestive tract of gilthead seabreams reared in floating cages in the Alghero Bay (North western Sardinia, Italy): preliminary results

Floris, Rosanna and Meloni, Gianni and Fois, Nicola and Pais, Antonio (2009) Bacterial flora associated with the digestive tract of gilthead seabreams reared in floating cages in the Alghero Bay (North western Sardinia, Italy): preliminary results. Italian Journal of Animal Science, Vol. 8 (Suppl. 2), p. 889. ISSN 1828-051X. Article.

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The intestinal microflora of fish is highly variable and can depend on many factors such as species, developmental stage, environmental conditions, trophic habits and, in the case of farmed fish, on rearing conditions (i.e., fish density, quality of rearing water, dietary regimen, etc.). Different studies showed that Gram-negative bacteria such as Enterobacteriaceae and the Vibrio-Aeromonas group dominate the fish intestine. However, since few studies have been carried out on the bacterial flora of intensively reared marine teleosts, the aim of the present study was to investigate the microflora associated with the digestive tract of Sparus aurata specimens reared in floating cages. A total of 30 gilthead seabreams (mean weight 324.9±61.4 g) starved for 48 hours was sampled in autumn 2008 from a fish farming facility located in the Alghero Bay (North western Sardinia, Italy: Lat 40°33’43.9’’N, Long 8°16’09.0’’E). The intestine between the pyloric caeca and the anus of each specimen was removed and analysed by means of conventional bacteriological techniques using five culture media: Plate Count Agar (PCA), Nutrient Agar (NA), Violet Red Bile Glucose Agar (VRBGA), Violet Red Bile Agar Mug (VRBA-MUG), and de Man-Rogosa-Sharpe (MRS) agar. Total viable counts on PCA and NA were quite similar, showing mean values of 126.7±109.0 colony forming units (cfu) per gram intestinal tissue (between 10.0 and 495.0 cfu/g) and 108.0±101.6 cfu/g (from nil to 350.0 cfu/g), respectively. The mean number of Enterobacteriaceae determined on VRBGA proved to be 46.3±42.8 cfu/g (from nil to 150 cfu/ g) and the VRBA-MUG mean count were 30.4±29.3 cfu/g (from nil to 95.0 cfu/g). No bacterial colonies were instead detected using the MRS medium. These results, although preliminary, evidenced a relative low number of bacteria associated with the digestive tract of the fish examined, thus indicating good hygienic conditions inside the cages as well as a suitable rearing density and a balanced diet for the seabreams.

Item Type:Article
ID Code:3034
Uncontrolled Keywords:Enterobacteriaceae, gilthead seabreams, aquaculture, Alghero, North western Sardinia
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/20 Zoocolture
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
002 Altri enti e centri di ricerca del Nord Sardegna > AGRIS Sardegna, Olmedo > Dipartimento per la ricerca nelle produzioni animali > Servizio risorse ittiche
Publisher:Avenue media on behalf of Scientific Association of Animal Production (ASPA)
Publisher Policy:Depositato per gentile concessione dell'ASPA
Additional Information:Abstract presentato al 18. Congresso nazionale ASPA, Palermo, 9-12 giugno 2009.
Deposited On:21 Sep 2009 10:06

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