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Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin

Dimauro, Corrado and Francesconi, Ana Helena Dias and Cappio Borlino, Aldo and McGuire, Mark A. (2009) Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin. Italian Journal of Animal Science, Vol. 8 (Suppl. 2), p. 78-80. ISSN 1828-051X. Article.

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Abstract

Administration of bovine somatotropin (bST) to lactating cows results in an increase in milk production from 10 to 15%. While physiological mechanisms involved in bST administration are well known, there is limited knowledge about the mechanisms that regulate the bST action at genetic level. For this reason, a microarray experiment was conducted to identify differentially expressed genes when bST is given to milking cows. Sixteen high-density microarrays for cattle, each containing 18,263 gene spots, were used. RNA was extracted from the mammary tissue of four lactating Holstein cows, five and two days before, and one and six days after bST administration. A total of 1,251 and 1,167 differentially expressed genes were detected for mean and median expression intensities, respectively. Only the 115 genes which were identified by both mean and median intensities were taken into account. These genes were grouped into 8 clusters according to changes in expression through time points.

Item Type:Article
ID Code:3013
Status:Published
Refereed:Yes
Uncontrolled Keywords:Microarray, somatotropin, cow
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Publisher:Avenue media on behalf of Scientific Association of Animal Production (ASPA)
ISSN:1828-051X
Publisher Policy:Depositato per gentile concessione dell'ASPA
Additional Information:Relazione presentata al 18. Congresso nazionale ASPA, Palermo, 9-12 giugno 2009.
Deposited On:18 Sep 2009 10:24

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