Zanetti, Stefania Anna Lucia and Angioi, A. and Fiori, Pier Luigi and Fadda, Giovanni (1994) Adhesion of mucoid uropathogenic strains of Pseudomonas aeruginosa to HEp-2 cells. New Microbiologica, Vol. 17 (4), p. 297-305. ISSN 1121-7138. Article.
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We studied a pili-independent adhesion mechanism to HEp-2 cells present in mucoid uropathogenic strains of Pseudomonas aeruginosa. Our data demonstrate that bacterial adhesion to HEp-2 cell surfaces is time dependent and that the phenotypes involved are influenced by bacterial growth conditions. Sonicated bacterial extracts competitively inhibit the adherence of homologous strains to HEp-2 cells. Adhesins that are heat and trypsin sensitive are located on the surface of the bacterial outer membrane. Immunogenic 55 kDa surface protein is required for the adherence to HEp-2 cell surfaces of non-piliated mucosal uropathogenic P. aeruginosa strains.
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