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Signal transduction in T lymphocytes - a comparison of the data from space, the free fall machine and the random positioning machine

Schwarzenberg, M. and Pippia, Proto Gavino and Meloni, Maria Antonia and Cossu, G. and Cogoli-Greuter, Marianne and Cogoli, Augusto (1999) Signal transduction in T lymphocytes - a comparison of the data from space, the free fall machine and the random positioning machine. Advances in Space Research, Vol. 24 (6), p. 793-800. ISSN 0273-1177. Article.

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DOI: 10.1016/S0273-1177(99)00075-7

Abstract

In this paper we discuss the effect of microgravity on T cells and we present the data of studies with two new machines for 0 g simulations. Several experiments in space show that mitogenic T cell activation is lost at 0 g. Immunocytochemistry indicates that such effect is associated with changes of the cytoskeleton. Biochemical studies suggest that the lack of expression of the interleukin-2 receptor is one of the major causes of the loss of activity. In fact, interleukin-2 is the third signal required for full activation. In order to deepen our investigations we are now working with the free-fall machine, FFM, invented by D. Mesland, and with the random positioning machine, RPM, or three-dimensional clinostat, developed by T. Hoson. The FFM produces periods of free-fall lasting approximately 800 ms followed by bounces of 15–30 g lasting 45–60 ms. The RPM eliminates the effect of gravity by rotating biological specimen randomly around two orthogonal axes. While the FFM failed to reproduce the results obtained with T lymphocytes in space, the data from the RPM are in good agreement with those in real microgravity. In fact, the inhibition of the mitotic index in the RPM is 89% compared to static controls. The RPM (as the FFM) can carry markedly larger specimen than the fast rotating clinostat and thus allows to conduct comprehensive studies to select suitable biological objects for further investigations in space.

Item Type:Article
ID Code:294
Status:Published
Refereed:Yes
Uncontrolled Keywords:Microgravity, T cells, cytoskeleton
Subjects:Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Pergamon
ISSN:0273-1177
Copyright Holders:© 1999 COSPAR, published by Elsevier Science
Deposited On:18 Aug 2009 10:01

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