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Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan

Sechi, Leonardo Antonio and Zanetti, Stefania Anna Lucia and Delogu, Giovanni and Montinaro, Barbara and Sanna, Alberto and Fadda, Giovanni (1996) Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan. Journal of Clinical Microbiology, Vol. 34 (7), p. 1825-1828. eISSN 1098-660X. Article.

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Abstract

The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.

Item Type:Article
ID Code:2934
Status:Published
Refereed:Yes
Uncontrolled Keywords:(GTG)5, fingerprinting method, M. tuberculosis
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-660X
Deposited On:09 Sep 2009 09:27

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