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Different strategies for molecular differentiation of Mycobacterium bovis strains isolated in Sardinia, Italy

Sechi, Leonardo Antonio and Leori, Guido and Lollai, Stefano and Duprè, Ilaria and Molicotti, Paola and Fadda, Giovanni and Zanetti, Stefania Anna Lucia (1999) Different strategies for molecular differentiation of Mycobacterium bovis strains isolated in Sardinia, Italy. Applied and Environmental Microbiology, Vol. 65 (4), p. 1781-1785. eISSN 1098-5336. Article.

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Abstract

Different genetic markers were used to analyze 22 Mycobacterium bovis strains isolated from cattle in Sardinia and one human isolate. IS6110 DNA fingerprinting differentiated the strains into six patterns, whereas with enterobacterial repetitive consensus sequence primers produced seven clusters. PCR ribotyping followed by digestion with HaeIII and PvuII produced five and seven patterns, respectively. PCR with the (GTG)5 oligonucleotide primer showed the best discriminatory power, generating eight clusters among the strains analyzed.

Item Type:Article
ID Code:2916
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium bovis, strain isolation, cattle, Sardinia
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-5336
Deposited On:04 Sep 2009 10:37

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