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Simple and rapid identification of different species of Mycobacteria by PCR

Sechi, Leonardo Antonio and Duprè, Ilaria and Sanguinetti, Maurizio and Fadda, Guido and Zanetti, Stefania Anna Lucia (1999) Simple and rapid identification of different species of Mycobacteria by PCR. Molecular and Cellular Probes, Vol. 13 (2), p. 141-146. ISSN 1096-1194. Article.

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DOI: 10.1006/mcpr.1999.0225

Abstract

A simple polymerase chain reaction (PCR) assay for rapid identification of different species of mycobacteria was developed. This PCR is based on the use of conserved sequences to amplify the genome of several mycobacterial species. The amplification patterns obtained were specific and reproducible for the species tested. In particular, we could identify Mycobacterium tuberculosis and Mycobacterium bovis (both produced the same pattern), Mycobacterium avium, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium chelonae, Mycobacterium peregrinum, Mycobacterium fortuitum, Mycobacterium gordonae and Mycobacterium smegmatis. Moreover, due to the numerous copies of the target sequences present in the genome, the PCR showed a very high level of sensitivity.

Item Type:Article
ID Code:2914
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacteria, identification, PCR, insertion sequences, RAPD
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Academic Press / Elsevier
ISSN:1096-1194
Deposited On:04 Sep 2009 09:29

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