UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Differentiation of Vibrio alginolyticus strains isolated from Sardinian waters by ribotyping and a new rapid PCR fingerprinting method

Zanetti, Stefania Anna Lucia and Deriu, Antonella and Duprè, Ilaria and Sanguinetti, Maurizio and Fadda, Giovanni and Sechi, Leonardo Antonio (1999) Differentiation of Vibrio alginolyticus strains isolated from Sardinian waters by ribotyping and a new rapid PCR fingerprinting method. Applied and Environmental Microbiology, Vol. 65 (5), p. 1871-1875. eISSN 1098-5336. Article.

Full text not available from this repository.

Alternative URLs:

Abstract

We investigated the usefulness of a novel PCR fingerprinting technique, based on the specific amplification of genomic regions, to differentiate 30 Vibrio alginolyticus strains isolated in Sardinian waters. The different profiles obtained were scanned and analyzed by a computer program in order to determine genetic relationships. The results were then compared with the patterns obtained by ribotyping with HindIII, KpnI, and XbaI restriction enzymes. PCR fingerprinting could differentiate the strains analyzed into 12 different patterns, whereas ribotyping with XbaI, which produced the highest number of patterns, generated only 7 different profiles. This study revealed the superior discriminative power of the proposed technique for the differentiation of related V. alginolyticus strains and the potential use of PCR fingerprinting in epidemiological studies.

Item Type:Article
ID Code:2913
Status:Published
Refereed:Yes
Uncontrolled Keywords:Vibrio alginolyticus, strains isolation, PCR fingerprinting, Sardinia
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-5336
Deposited On:04 Sep 2009 09:09

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page