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Identification of HIV patients with active pulmonary tuberculosis using urine based polymerase chain reaction assay

Aceti, Antonio and Zanetti, Stefania Anna Lucia and Mura, Maria Stella Anna and Sechi, Leonardo Antonio and Turrini, Francesco Michelangelo and Saba, Franca and Babudieri, Sergio and Mannu, Franca and Fadda, Giovanni (1999) Identification of HIV patients with active pulmonary tuberculosis using urine based polymerase chain reaction assay. Thorax, Vol. 54 (2), p. 145-146. eISSN 1468-3296. Article.

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Abstract

BACKGROUND Despite the increased dissemination of tuberculosis among HIV infected patients, the diagnosis is difficult to establish. Traditional microbiological methods lack satisfactory sensitivity. We have developed a highly sensitive and specific nested polymerase chain reaction (PCR) capable of detecting Mycobacterium tuberculosis DNA in urine specimens and have used this test to examine urine specimens from HIV patients with active pulmonary tuberculosis.
METHODS Urine specimens from 13 HIV infected patients with microbiologically proven active pulmonary tuberculosis, 10 AIDS patients with non-tuberculous mycobacterial infection (documented by blood culture), 53 AIDS patients with no evidence of mycobacterial disease, and 80 healthy subjects (25 with positive skin test to purified protein derivative) were tested for M tuberculosis using PCR, acid fast staining (AFS), and culture.
RESULTS Of the urine specimens from patients with active tuberculosis, all tested positive by PCR, two by culture, and none by AFS. No reactivity was observed in urine specimens from patients with non-tuberculous mycobacterial infection. Of the 53 AIDS patients without mycobacterial infection, one had a positive urine PCR. Normal subjects were all negative.
CONCLUSIONS Urine based nested PCR for M tuberculosis may be a useful test for identifying HIV patients with pulmonary tuberculosis.

Item Type:Article
ID Code:2908
Status:Published
Refereed:Yes
Uncontrolled Keywords:Tuberculosis, urine, polymerase chain reaction, AIDS
Subjects:Area 06 - Scienze mediche > MED/17 Malattie infettive
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Malattie infettive e parassitarie
Publisher:BMJ Publishing Group
eISSN:1468-3296
Deposited On:04 Sep 2009 11:00

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