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Distribution of a specific 500-base-pair fragment in Mycobacterium bovis isolates from Sardinian cattle

Sechi, Leonardo Antonio and Duprè, Ilaria and Leori, Guido and Fadda, Giovanni and Zanetti, Stefania Anna Lucia (2000) Distribution of a specific 500-base-pair fragment in Mycobacterium bovis isolates from Sardinian cattle. Journal of Clinical Microbiology, Vol. 38 (10), p. 3837-3839. eISSN 1098-660X. Article.

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Abstract

Amplification of a specific, 500-bp fragment from Mycobacterium bovis isolates and use of the fragment to differentiate between Mycobacterium tuberculosis and M. bovis was previously reported (J. G. Rodriguez, G. A. Meja, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131-2138, 1995). In the present study, 30 M. bovis isolates from Sardinian cattle were examined for the presence of this 500-bp fragment; 4 of the 30 isolates lacked the fragment. This result indicates that identification of M. bovis strains by amplification of the 500-bp sequence may lead to false-negative results.

Item Type:Article
ID Code:2905
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium bovis, infection, cattle, Sardinia
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:American Society for Microbiology
eISSN:1098-660X
Deposited On:03 Sep 2009 11:16

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