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Rapid identification of cutaneous infections by nontubercular mycobacteria by polymerase chain reaction-restriction analysis length polymorphism of the hsp65 gene

Ena, Pasquale and Sechi, Leonardo Antonio and Saccabusi, Stanislao and Molicotti, Paola and Lorrai, Maria Paola and Siddi, Massimiliano and Zanetti, Stefania Anna Lucia (2001) Rapid identification of cutaneous infections by nontubercular mycobacteria by polymerase chain reaction-restriction analysis length polymorphism of the hsp65 gene. International Journal of Dermatology, Vol. 40 (8), p. 495-499. eISSN 1365-4632. Article.

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Abstract

Background. Nontubercular mycobacteria (NTM) may cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. This study involved eight patients (four men and four women) with primary cutaneous infections caused by NTM; the skin lesions included dermo-hypodermal abscesses, suppurative granulomas, and papulonodules localized on the legs, arms, hands, and face. The general condition of the patients was relatively good and they were not immunosuppressed.
Methods. All samples were processed with standard methods and the isolates were identified by pattern restriction analysis after polymerase chain reaction (PCR-PCA) amplification of the heat shock protein of 65 kDa.
Results. In this way, we were able to identify three Mycobacterium chelonae strains, two Mycobacterium marinum, two Mycobacterium fortuitum, and one Mycobacterium avium. The lesions disappeared in 3 or 4 weeks after treatment with two or more antimicrobials.
Conclusions. For a correct diagnosis of cutaneous infection by NTM, demonstrating the presence of mycobacteria is essential; routinely available techniques lack sensitivity and are extremely tedious; often mycobacteria are not seen after acid-fast stain. We used PCR-PCA to identify mycobacteria grown in liquid media; the time of identification of mycobacteria was shortened relative to conventional methods.

Item Type:Article
ID Code:2890
Status:Published
Refereed:Yes
Uncontrolled Keywords:Nontubercular mycobacteria (NTM), cutaneous infections, diagnosis
Subjects:Area 06 - Scienze mediche > MED/35 Malattie cutanee e veneree
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Dermatologia
Publisher:Blackwell / Wiley
eISSN:1365-4632
Publisher Policy:Journal compilation © 2009 International Society of Dermatology
Additional Information:Pubblicato online il 7 luglio 2008.
Deposited On:02 Sep 2009 09:40

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