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Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells

Sciola, Gian Luigi and Cogoli-Greuter, Marianne and Cogoli, Augusto and Spano, Alessandra and Pippia, Proto Gavino (1999) Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells. Advances in Space Research, Vol. 24 (6), p. 801-805. ISSN 0273-1177. Article.

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DOI: 10.1016/S0273-1177(99)00078-2

Abstract

The effects of microgravity on Jurkat cells — a T-lymphoid cell line — was studied on a sounding rocket flight. An automated pre-programmed instrument permitted the injection of fluorescent labelled concanavalin A (Con A), culture medium and/or fixative at given times. An in-flight 1 g centrifuge allowed the comparison of the data obtained in microgravity with a 1 g control having the same history related to launch and re-entry. After flight, the cells fixed either at the onset of microgravity or after a 12 minute incubation time with fluorescent concanavalin A were labelled for vimentin and actin and analysed by fluorescence microscopy. Binding of Con A to Jurkat cells is not influenced by microgravity, whereas patching of the Con A receptors is significantly lower. A significant higher number of cells show changes in the structure of vimentin in microgravity. Most evident is the appearance of large bundles, significantly increased in the microgravity samples. No changes are found in the structure of actin and in the colocalisation of actin on the inner side of the cell membrane with the Con A receptors after binding of the mitogen.

Item Type:Article
ID Code:289
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cytoskeletal structures, microgravity, mitogen
Subjects:Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Pergamon
ISSN:0273-1177
Copyright Holders:© 1999 COSPAR, published by Elsevier Science
Deposited On:18 Aug 2009 10:01

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