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Strain variation in Mediterranean and Red Sea Mycobacterium marinum isolates

Sechi, Leonardo Antonio and Colorni, Angelo and Duprè, Ilaria and Molicotti, Paola and Fadda, Giovanni and Zanetti, Stefania Anna Lucia (2002) Strain variation in Mediterranean and Red Sea Mycobacterium marinum isolates. New Microbiologica, Vol. 25 (3), p. 351-356. ISSN 1121-7138. Article.

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Four different PCR fingerprinting techniques were tested to distinguish possible strain variations in fourteen Mycobacterium marinum isolates, thirteen from Mediterranean and Red Sea fishes and one from a patient in Sardinia, Italy. PCR ribotyping and ERIC (enterobacterial repetitive consensus sequences)-PCR were found to be non-discriminative, whereas IS (insertion sequences)-PCR and GTG (GTG sequences repeats)-PCR could distinguish the clinical isolate from the piscine isolates, two Italian piscine isolates from all other isolates, but not the Greek isolates from the Israeli isolates. Our results indicate that GTG-PCR and IS-PCR have superior discriminative properties and are thus useful molecular tools for epidemiological studies of M. marinum.

Item Type:Article
ID Code:2884
Uncontrolled Keywords:Mycobacterium marinum, PCR fingerprinting, Mediterranean Sea, Red Sea
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Edizioni internazionali
Deposited On:01 Sep 2009 13:32

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