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Rapid detection and identification of Mycobacterium tuberculosis by Real Time PCR and Bactec 960 MIGT

Ortu, Silvia and Molicotti, Paola and Sechi, Leonardo Antonio and Pirina, Pietro and Saba, Franca and Vertuccio, Cono and Deriu, Antonella and Maida, Ivana Rita and Mura, Maria Stella Anna and Zanetti, Stefania Anna Lucia (2006) Rapid detection and identification of Mycobacterium tuberculosis by Real Time PCR and Bactec 960 MIGT. New Microbiologica, Vol. 29 (1), p. 75-80. ISSN 1121-7138. Article.

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Abstract

We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.

Item Type:Article
ID Code:2877
Status:Published
Refereed:Yes
Uncontrolled Keywords:Real Time quantitative PCR, Mycobacterium tuberculosis, non-tuberculous mycobacteria, molecular diagnosis of tuberculosis, insertion sequence IS6110
Subjects:Area 06 - Scienze mediche > MED/17 Malattie infettive
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Malattie infettive e parassitarie
Publisher:Edizioni internazionali
ISSN:1121-7138
Deposited On:31 Aug 2009 16:41

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