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Immunogenicity and cytoadherence of recombinant heparin binding haemagglutinin (HBHA) of Mycobacterium avium subsp. paratuberculosis: functional promiscuity or a role in virulence?

Sechi, Leonardo Antonio and Ahmed, Niyaz and Felis, Giovanna E. and Duprè, Ilaria and Cannas, Sara and Fadda, Giovanni and Bua, Alessandra and Zanetti, Stefania Anna Lucia (2006) Immunogenicity and cytoadherence of recombinant heparin binding haemagglutinin (HBHA) of Mycobacterium avium subsp. paratuberculosis: functional promiscuity or a role in virulence? Vaccine, Vol. 24 (3), p. 236-243. eISSN 1358-8745. Article.

Full text not available from this repository.

DOI: 10.1016/j.vaccine.2005.11.030

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's Disease, a chronic granulomatous enteritis of ruminants. Recently, an association of MAP bacilli with Crohn's disease in humans has been proposed. Due to genetic similarities and serological cross-reactivity of the M. avium complex with other mycobacteria, functional analysis of species-specific proteins may allow new insights into the pathogenesis of mycobacterial diseases. We report production and molecular characterization of the recombinant HBHA from the MAP complex bacilli. The HBHA was expressed in Escherichia coli and Mycobacterium smegmatis using efficient expression vector systems. The recombinant HBHA was found to be immunogenic and therefore induced antibody responses in cattle against the MAP bacilli with a possible cross reactivity with M. bovis infection. The MAP complex HBHA was thus found to be a target of the host humoral responses in Johne's disease. The recombinant HBHA protein was also found to be adherent to the Caco2 cell lines in-vitro, a significant observation to understand possible virulence mechanisms. Since M. tuberculosis HBHA was earlier shown to be involved in dissemination of the tubercle bacilli, the immunogenicity and cytoadherent nature of this MAP protein possibly suggests functional promiscuity.

Item Type:Article
ID Code:2876
Status:Published
Refereed:Yes
Uncontrolled Keywords:Immunogenicity, recombinant heparin binding haemagglutinin, Mycobacterium avium subsp. paratuberculosis
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier
eISSN:1358-8745
Deposited On:31 Aug 2009 16:01

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