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PCR-based detection of the Mycobacterium tuberculosis complex in urine of HIV-infected and uninfected pulmonary and extrapulmonary tuberculosis patients in Burkina Faso

Torrea, Gabriela and Van de Perre, Philippe and Ouedraogo, Martial and Zougba, Alain and Sawadogo, Adria and Dingtoumda, Benoït and Diallo, Boukari and Defer, Marie Christine and Sombié, Issiaka and Zanetti, Stefania Anna Lucia and Sechi, Leonardo Antonio (2005) PCR-based detection of the Mycobacterium tuberculosis complex in urine of HIV-infected and uninfected pulmonary and extrapulmonary tuberculosis patients in Burkina Faso. Journal of Medical Microbiology, Vol. 54 (1), p. 39-44. eISSN 1473-5644. Article.

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DOI: 10.1099/jmm.0.45688-0

Abstract

To evaluate a one-tube nested PCR-based analysis of urine for diagnosing pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) in Bobo-Dioulasso, Burkina Faso, a prospective analysis of urine samples from HIV- and non-HIV-infected adults with PTB and EPTB (case patients) and with pathology other than tuberculosis (TB) (control patients) was performed. Three groups of patients were classified as microbiological-positive and -negative PTB and EPTB on the basis of clinical signs and microbiological results. Urine from patients was analysed using the DNA extraction and Sechi's methods, both modified, for the detection of Mycobacterium tuberculosis. The sensitivity, specificity, positive predictive value and negative predictive value were calculated. The sensitivity of the test for the microbiological-positive PTB, microbiological-negative PTB and EPTB was 40.5 % (88/217), 66.7 % (20/30) and 57.1 % (48/84), respectively. The specificity was 98.2 %. Differences were observed in the two populations infected and not infected by HIV. This method is not appropriate for detection of new TB cases in the routine laboratory, but it can be useful for cases where the clinical and bacteriological diagnosis of TB is not conclusive.

Item Type:Article
ID Code:2874
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium tuberculosis, HIV infection, Burkina Faso
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Society for General Microbiology
eISSN:1473-5644
Deposited On:31 Aug 2009 13:48

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