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Mycobacterium tuberculosis molecular evolution in western Mediterranean islands of Sicily and Sardinia

Sola, Christophe and Ferdinand, Séverine and Sechi, Leonardo Antonio and Zanetti, Stefania Anna Lucia and Martial, Dominique and Mammina, Caterina and Nastasi, Antonino and Fadda, Giovanni and Rastogi, Nalin (2005) Mycobacterium tuberculosismolecular evolution in western Mediterranean islands of Sicily and Sardinia. Infection, Genetics and Evolution, Vol. 5 (2), p. 145-156. ISSN 1567-1348. Article.

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DOI: 10.1016/j.meegid.2004.08.003

Abstract

In this study, a total of 204 Mycobacterium tuberculosis DNAs from Sicily (n = 144) and Sardinia (n = 60) were studied by three genotyping methods. Results were analyzed both within and across islands, to define the phylogeographical specificities of the genotypes, look for their diversity and infer a molecular evolutionary scenario. A strong link between geography and tuberculosis genotypes was observed in Sardinia. The results were also matched against a world-wide genetic diversity database to compare the population structure of the tubercle bacilli in the islands. Eight common genotypes between Sicily, Sardinia and continental Italy were found which underlines the influences of the Italian mainland on the population structure on the islands and vice versa. A unified evolutionary scenario of M. tuberculosis evolution was built using numerical taxonomy and maximum parsimony (MP) methods. The finding of multiple families of M. tuberculosis strains (S, T, LAM, Haarlem), their presumed links with the major genetic groups (MGG) of M. tuberculosis complex, supports the view of independent introduction of several ancestral genotypes in Sicily and in Sardinia. We conclude that the two PCR-based genotyping combination (spoligotyping–VNTR) is an excellent tool to reconstruct M. tuberculosis phylogeny, that may be used to construct global and local evolutionary scenarios of the M. tuberculosis complex. The results obtained are paradigmatic of the complex interplay that exists between epidemic dynamics and evolutionary genetics of M. tuberculosis.

Item Type:Article
ID Code:2872
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycobacterium tuberculosis complex, molecular evolution, Sicily, Sardinia, genetics
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier
ISSN:1567-1348
Deposited On:31 Aug 2009 12:54

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