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Aeromonas caviae keratitis associated with contact lens wear

Pinna, Antonio <1963- > and Sechi, Leonardo Antonio and Zanetti, Stefania Anna Lucia and Usai, Donatella and Carta, Francesco (2004) Aeromonas caviae keratitis associated with contact lens wear. Ophthalmology, Vol. 111 (2), p. 348-351. ISSN 0161-6420. Article.

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DOI: 10.1016/j.ophtha.2003.05.012

Abstract

Objective: We report the first case of bilateral contact lens–related Aeromonas caviae keratitis associated with A. caviae contamination of the contact lens case. The presence of virulence factors produced by Aeromonas species was also investigated.
Design: Case report.
Intervention and Testing: Conjunctival swabs and corneal scrapings from both eyes were inoculated for culture. The contact lens case was also cultured. The isolate was analyzed for the presence of virulence properties, such as gelatinase and protease production. The presence of virulence genes, such as the cytolytic enterotoxin (AHCYTOEN) and type IV Aeromonas pilus (tap) genes, was investigated using polymerase chain reaction. The susceptibility of A. caviae to 6 commercial contact lens disinfecting solutions was tested.
Main Outcome Measures: Culture results, protease activity, and gelatinase production were analyzed. Polymerase chain reaction amplification products were visualized in ethidium bromide–stained agarose gel. Bacterial growth after exposure to contact lens disinfecting solutions was assessed.
Results: Aeromonas caviae was grown bilaterally from the conjunctiva, cornea, and contact lens case. The organism showed protease and gelatinase production. Polymerase chain reaction amplification revealed that the A. caviae strain contained the AHCYTOEN and tap virulence genes. Incubation for the minimum recommended time with all tested disinfecting solutions was effective in killing A. caviae
Conclusions: Aeromonas caviae should be considered a possible etiologic agent of contact lens–associated keratitis. The presence of virulence factors may be important in determining corneal infection. Commercial contact lens disinfecting solutions, along with proper lens case hygiene, may be effective in preventing A. caviae keratitis in soft contact lens wearers.

Item Type:Article
ID Code:2857
Status:Published
Refereed:Yes
Uncontrolled Keywords:Corneal infection, AHCYTOEN gene, keratitis
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Oftalmologia, Otorinolaringoiatria e Urologia
Publisher:Elsevier Science
ISSN:0161-6420
Copyright Holders:© 2004 by the American Academy of Ophthalmology
Deposited On:01 Sep 2009 16:49

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