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Fate of transformed Trichoderma harzianum in the phylloplane of tomato plants

Migheli, Quirico and Herrera-Estrella, A. and Avataneo, M. and Gullino, Maria Lodovica (1994) Fate of transformed Trichoderma harzianum in the phylloplane of tomato plants. Molecular Ecology, Vol. 3 (2), p. 153-159. eISSN 1365-294X. Article.

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DOI: 10.1111/j.1365-294X.1994.tb00116.x


A propiconazole-resistant Trichoderma harzianum strain with high phylloplane survival capability was transformed with the E. coli hygromycin B phosphotransferase gene (hph), coding for hygromycin B resistance. Four transformants were analysed for survival ability on the phylloplane of tomato plants grown under glasshouse conditions in comparison with their prototype and a yellow, hygromycin B-sensitive mutant. Over 2 weeks, the four transformants showed higher survival rates in comparison with the wild-type strain. The yellow mutant TF3/973 did not significantly differ in survival from the transformants. Both hygromycin B resistance and mitotic stability of transformants were evaluated during growth in vitro and after reisolation from tomato phylloplane. Hybridization patterns with the complete plasmid indicated that all four transformants were mitotically stable after several rounds of vegetative growth without selective pressure and during 2 weeks on tomato plants. None of the transformants had lost the ability to grow in the presence of both propiconazole and hygromycin B after growth under the same conditions. The results are discussed in relation to risk assessment of the release of transgenic fungi

Item Type:Article
ID Code:275
Uncontrolled Keywords:Biological control, Botrytis cinerea, genetic transformation, mitotic stability, phylloplane survival, tomato, Trichoderma harzianum
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Deposited On:18 Aug 2009 10:01

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