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The Diagnosis of Echinococcus granulosus in dogs

Varcasia, Antonio and Garippa, Giovanni and Scala, Antonio (2004) The Diagnosis of Echinococcus granulosus in dogs. Parassitologia, Vol. 46 (4), p. 409-412. ISSN 0048-2951. Article.

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Abstract

The problem of diagnosing Echinococcus granulosus in dogs has still only been partially resolved, even after the advent of biotechnology. The eggs of taeniid Cestoda are extremely similar, and thus identification by microscopic examination of the faeces is risky and non-specific. For this reason, Echinococcus granulosus was traditionally diagnosed in dogs ante mortem after an arecoline hydrobromate purge. The faeces were examined macro and microscopically to establish if the adult tapeworm or its proglottids were present. Although this method is 100% specific, it is bio-hazardous and time-consuming, requires trained personnel, and its sensitivity varies. In the 1990s copro-antigens were discovered and characterised. These are released by the adult worm in the faeces. This made it possible to use enzyme-linked immune-adsorbent assay (ELISA) for in vitam diagnosis of Echinococcus granulosus. In recent years several PCR protocols have been published on the identification of Echinococcus granulosus DNA from eggs or from adult parasites and new ways of diagnosing this cestode have been developed.

Item Type:Article
ID Code:2678
Status:Published
Refereed:Yes
Uncontrolled Keywords:Diagnosis, dogs, echinococcosis, copro-antigens, PCR
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/06 Parassitologia e malattie parassitarie degli animali
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:For the University of Rome "La Sapienza" by Lombardo Editore
ISSN:0048-2951
Publisher Policy:Depositato per gentile concessione del Dipartimento di Scienze di sanità pubblica, Sezione di parassitologia "Ettore Bicocca", Università di Roma "La Sapienza"
Additional Information:Relazione presentata al First National Congress of Hydatidology, Sassari, Italy, October 7-8, 2004.
Deposited On:18 Aug 2009 10:08

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