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In vitro acanthamoebicidal activity of a killer monoclonal antibody and a synthetic peptide

Fiori, Pier Luigi and Mattana, Antonella and Dessì, Daniele and Conti, Stefania and Magliani, Walter and Polonelli, Luciano (2006) In vitroacanthamoebicidal activity of a killer monoclonal antibody and a synthetic peptide. Journal of Antimicrobial Chemotherapy, Vol. 57 (5), p. 891-898. eISSN 1460-2091. Article.

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DOI: 10.1093/jac/dkl051

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Objectives: To evaluate the in vitro microbicidal activity against Acanthamoeba castellanii of a murine monoclonal anti-idiotypic antibody (KTmAb) and a synthetic killer mimotope (KP), which mimic a yeast killer toxin (KT) characterized by a wide spectrum of antimicrobial activity through interaction with specific cell wall receptors, mainly constituted by ß-glucans. Methods: Amoebicidal activity was investigated after incubation of trophozoites under different experimental conditions with laminarinase, KTmAb, KP and a scrambled decapeptide (SP). To confirm the specific interaction of KP with ß-glucans, the experiments were also carried out in the presence of laminarin (ß1-3-glucan) or pustulan (ß1-6-glucan); both glucan molecules were co-incubated with KP or SP. Results: KTmAb and KP exhibited a time-dependent killing activity, in comparison with SP or heat-inactivated KTmAb; this activity was completely abolished by pre-incubation with laminarin, but not by pustulan. Notably, in vitro amoebicidal activity was observed in the presence of laminarinase, an enzyme that specifically hydrolyses ß-glucans. Furthermore, KP specifically inhibited the growth of Acanthamoeba on infected contact lenses and the remaining adherent KP-treated trophozoites appeared strongly damaged. Conclusions: The results indicate that the expression of ß1-3-glucan receptors in the cell membrane is probably modulated during cell growth of A. castellanii and is critical for the killing activity of KT-like molecules. Our data confirm the broad antimicrobial spectra of KTmAb and KP, emphasize the crucial role of ß1-3-glucan in microbial physiology and suggest the potential use of KTmAb and KP in the prevention and therapy of Acanthamoeba infections or in preventing Acanthamoeba contamination during storage of contact lenses.

Item Type:Article
ID Code:263
Uncontrolled Keywords:Acanthamoeba, antimicrobial peptides, anti-idiotypic antibodies, antibody-derived peptides, killer toxins
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy
Copyright Holders:© The Author 2006
Additional Information:Pubblicato online il 26 febbraio 2006.
Deposited On:18 Aug 2009 10:01

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