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Electrophoretic karyotype variation among pathotypes of Fusarium oxysporum f.sp. dianthi

Migheli, Quirico and Berio, T. and Gullino, Maria Lodovica and Garibaldi, Angelo (1995) Electrophoretic karyotype variation among pathotypes of Fusarium oxysporum f.sp. dianthi. Plant Pathology, Vol. 44 (2), p. 308-315. ISSN 1365-3059. Article.

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DOI: 10.1111/j.1365-3059.1995.tb02782.x


Karyotype analysis by pulsed-field gel electrophoresis was applied to characterize isolates of Fusarium oxysporum f.sp. dianthi, the causal agent of Fusarium wilt on carnation. Eleven distinct chromosomal DNA patterns were detected among 38 pathogenic isolates, and the total genome size was estimated to range from 23·7 to 36·4 Mb. Except for isolates belonging to pathotypes 2 and 4, all members of the same pathotype shared overlapping electrophoretic karyotypes. Karyotypes of isolates assigned to pathotypes 1 and 8 showed a high degree of similarity, in accordance with VCG and RFLP analysis. The same electrophoretic karyotype was also shared by members of pathotypes 2 and 5, thus confirming results obtained by both VCG and RFLP grouping, A single representative of pathotype 6, previously confined to the same VCG and RFLP group as pathotypes 2 and 5, had a slightly different chromosomal pattern. Isolates assigned to pathotype 4 showed four related karyotypes which partially differed in both the number and size of chromosomal bands. However, all strains assigned to this pathotype shared a basic profile of nine chromosomal bands, while two low-molecular-weight bands were present or absent. The findings are discussed with regard both to the suitability of race distinction in the case of the special form dianthi of F. oxysporum and to the use of karyotype analysis by PFGE as a tool for the study of the population genetics of this fungus

Item Type:Article
ID Code:262
Uncontrolled Keywords:Karyotype, gel electrophoresis, genome, isolate, Pathotype, restriction fragment length polymorphism, physiologic race, genetic variability, chromosome DNA, molecular biology, population genetics, Fusarium oxysporum, fungi imperfecti, Thallophyta, plant pathogen, nucleic acid
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:British Society for Plant Pathology
Publisher Policy:La versione definitiva è disponibile alla pagina www3.interscience.wiley.com/
Deposited On:18 Aug 2009 10:01

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