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Determination of creatinine in human serum by short-end injection capillary zone electrophoresis

Zinellu, Angelo and Carru, Ciriaco and Usai, Maria Franca and Sotgia, Salvatore and Deiana, Luca (2004) Determination of creatinine in human serum by short-end injection capillary zone electrophoresis. Electrophoresis, Vol. 25 (7-8), p. 1096-1101. eISSN 1522-2683. Article.

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DOI: 10.1002/elps.200305793

Abstract

A new ultra-rapid free-solution capillary zone electrophoresis method to measure serum creatinine is presented. Procedural parameters such as injection mode, concentration and pH of phosphate running buffer and acidic deproteinization of serum samples were investigated. Short-end injection permits a decrease of the analysis time by injecting samples at the outlet end of a silica capillary closest to the detection window, so reducing the migration distance. Thus, when a capillary with an effective length of 10.2 cm and a 40 mmol/L sodium phosphate buffer pH 2.35 was used, the obtained migration time of the creatinine peak was the shortest never described before, about 1.1 min. These conditions give a good reproducibility of the migration times (coefficient of variation, CV% < 0.5) and the peak areas (CV% < 2.8). Intra- and interassay CV were 3.06 and 6.26%, respectively, and analytical recovery was 99.4%. We compared our proposed method to Jaffé colorimetric assay, by measuring serum creatinine in 128 normal subjects. The obtained data were analyzed by the Passing and Bablok regression and Bland-Altman test. Creatinine concentration in healthy subjects was also used to investigate on its relationships with plasma thiols levels.

Item Type:Article
ID Code:2527
Status:Published
Refereed:Yes
Uncontrolled Keywords:Capillary electrophoresis, creatinine, reversed polarity, short-end injection, thiols
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Wiley
eISSN:1522-2683
Deposited On:18 Aug 2009 10:08

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