Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
A low oxygen atmosphere during IVF accelerates the kinetic of formation of in vitro produced ovine blastocysts

Leoni, Giovanni Giuseppe and Rosati, Irma and Succu, Sara and Bogliolo, Luisa and Bebbere, Daniela and Berlinguer, Fiammetta and Ledda, Sergio and Naitana, Salvatore (2007) A low oxygen atmosphere during IVF accelerates the kinetic of formation of in vitro produced ovine blastocysts. Reproduction in Domestic Animals, Vol. 42 (3), p. 299-304. eISSN 1439-0531. Article.

Full text not available from this repository.

DOI: 10.1111/j.1439-0531.2006.00783.x


Among the factors that affect in vitro embryo development, oxygen atmosphere is considered to be of great influence. In this study, we evaluated the influence of two different oxygen atmospheres during in vitro fertilization (IVF) of ovine oocytes on their developmental capacity and quality assessed by cryotolerance. Cumulus oocyte complexes derived from ovaries of slaughtered sheep were matured in vitro and subsequently fertilized under low (5%) or high (20%) oxygen atmospheres, and cultured in SOF + aa + 0.4% BSA in 5% CO2 and 5% O2 up to blastocyst stage. The cleavage rates obtained in the fertilization system at 20% O2 were significantly higher than those obtained in the 5% O2 fertilization system (61.2% vs 50.8%; p < 0.01). The distribution of cleaved oocytes at 22, 26 and 40 h of culture intervals was not different in the low or high O2 atmosphere (31.4%, 26.4% and 42.1% vs 28.0%, 29.3% and 42.7% respectively). Blastocysts output on the 6th day post-fertilization (dpf) was significantly higher when oocytes were fertilized under 5% O2 concentration (63.04% in 5% O2 vs 47.36% in 20% O2), while on the 7th dpf the higher number of blastocysts was obtained in the 20% O2 system (35.10%.in 20% O2 vs 26.09% in 5% O2). After vitrification no differences were observed between low or high oxygen atmosphere in the viability rates of blastocysts obtained on day 6 (93.6% vs 96.5%), on day 7 (46.3% vs 41.7%) and on day 8 (11.1% vs 6.6%). After differential staining, no significant differences were observed in the total cell number and inner cell mass and trophoblastic cells ratio of blastocysts produced on 6 dpf (189.6 ± 51.3 and 0.260 ± 0.07 vs 223.3 ± 45.6 and 0.277 ± 0.09), on 7 dpf (168.3 ± 25.1 and 0.316 ± 0.06 vs 172.1 ± 33,6 and 0.320 ± 0.06) and on 8 dpf (121.2 ± 23,8 and 0.302 ± 0.03 vs 117.0 ± 35.1 and 0.313 ± 0.04) under low or high oxygen atmosphere respectively). In conclusion, our data suggest that low oxygen atmosphere during IVF affects positively the production of high quality ovine blastocysts.

Item Type:Article
ID Code:2469
Uncontrolled Keywords:IVF, ovine blastocysts, embryo development
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
Copyright Holders:© 2007 The Authors, Journal compilation © 2007 Blackwell
Deposited On:18 Aug 2009 10:07

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page