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A new HPLC method for serum neopterin measurement and relationships with plasma thiols levels in healthy subjects

Carru, Ciriaco and Zinellu, Angelo and Sotgia, Salvatore and Serra, Roberta and Usai, Maria Franca and Pintus, Gianfranco and Pes, Giovanni Mario and Deiana, Luca (2004) A new HPLC method for serum neopterin measurement and relationships with plasma thiols levels in healthy subjects. Biomedical Chromatography, Vol. 18 (6), p. 360-366. eISSN 1099-0801. Article.

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DOI: 10.1002/bmc.325

Abstract

Neopterin, a pyrazinopyrimidine compound, serves as a marker of cellular immune system activation, and it can be used as a prognostic predictor for certain types of diseases. We propose a new simple HPLC method to measure serum neopterin with highly sensitive fluorimetric detection. After TCA serum protein precipitation, the supernatant was diluted five times, injected into a C18 reversed-phase column and eluted at a flow rate of 1.5 mL/min by an isocratic water-acetonitrile (99:1) mobile phase. The natural fluorescence of the molecule was detected at excitation wavelength 353 nm and emission 438 nm. In these conditions the neopterin retention time was about 4 min. Our proposed method was compared with a validated chromatographic separation, and the obtained data of the serum neopterin from 35 healthy volunteers were analysed by Passing-Bablok regression and Bland-Altman test. Neopterin measurement in healthy subjects was also employed to investigate on its potential relationships with plasma thiols levels.

Item Type:Article
ID Code:2452
Status:Published
Refereed:Yes
Uncontrolled Keywords:high-performance liquid chromatography, neopterin, thiols, homocysteine, cysteine, cardiovascular risk
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Laboratorio analisi cliniche Meridiana Srl, Sassari
Publisher:Wiley
eISSN:1099-0801
Deposited On:18 Aug 2009 10:07

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