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Assay for the simultaneous determination of guanidinoacetic acid, creatinine and creatine in plasma and urine by capillary electrophoresis UV-detection

Zinellu, Angelo and Sotgia, Salvatore and Zinellu, Elisabetta and Chessa, Roberto and Deiana, Luca and Carru, Ciriaco (2006) Assay for the simultaneous determination of guanidinoacetic acid, creatinine and creatine in plasma and urine by capillary electrophoresis UV-detection. Journal of Separation Science, Vol. 29 (5), p. 704-708. eISSN 1615-9314. Article.

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DOI: 10.1002/jssc.200500428

Abstract

Guanidinoacetic acid (GAA) measurement has recently become of great interest for the diagnosis of creatine (Cn) metabolism disorders, and research calls for rapid and inexpensive methods for its detection in plasma and urine in order to assess a large number of patients. We propose a new assay for the measurement of GAA by a simple CZE UV-detection without previous sample derivatization. Plasma samples were filtered by Microcon-10 microconcentrators and directly injected into the capillary, while for urine specimens a simple water dilution before injection was needed. A baseline separation was obtained in less than 8 min using a 60.2 cm×75 m uncoated silica capillary, 75 mmol/L Tris-phosphate buffer pH 2.25 at 15°C. The performance of the developed method was assessed by measuring plasma creatinine and Cn in 32 normal subjects and comparing the data obtained by the new method with those found with the previous CE assay. Our new method seems to be an inexpensive, fast and specific tool to assess a large number of patients both in clinical and in research laboratories.

Item Type:Article
ID Code:2440
Status:Published
Refereed:Yes
Uncontrolled Keywords:Capillary electrophoresis, creatine, creatinine, guanidinoacetic acid
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Wiley
eISSN:1615-9314
Deposited On:18 Aug 2009 10:07

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