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Genetic traceability of the geographical origin of typical Italian water buffalo Mozzarella cheese: a preliminary approach

Bonizzi, Ivan and Feligni, Maria and Aleandri, Riccardo and Enne, Giuseppe (2007) Genetic traceability of the geographical origin of typical Italian water buffalo Mozzarella cheese: a preliminary approach. Journal of Applied Microbiology, Vol. 102 (3), p. 667-673. eISSN 1365-2672. Article.

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DOI: 10.1111/j.1365-2672.2006.03131.x


Aims: To distinguish Italian Protected Designation of Origin (PDO) water buffalo Mozzarella from different producers on a molecular basis in relation to the place of manufacturing within the production district, and to develop a tool for genetic traceability of typical dairy products.
Methods and Results: Microbial DNA was isolated from Mozzarella's governing liquid to amplify the whole microflora's ribosomal 16S–23S internal transcribed spacers (ITS)-PCR fingerprinting by means of an original primer pair. Phylogenetic distance analyses were performed on the obtained electrophoretic band patterns by maximum parsimony and neighbour-joining tree construction algorithms for discrete binary data, using a conventional bootstrap resampling test. The observed band profiles showed high repeatability and specificity, allowing unambiguous distinction of each sample; phylogenetic analyses yielded the same tree topology with good strength of nodal support. Moreover, a relationship between the genetic distances among samples and the actual geographical ones separating the respective producing dairies was observed.
Conclusions: The genetic diversity of PDO water buffalo Mozzarella's microflora, observed by ITS-PCR fingerprinting, can be exploited to discriminate cheeses from differently located dairies.
Significance and Impact of the Study: Given the increasing importance of food traceability for safety, quality and typicalness issues, the ITS-PCR fingerprinting protocol described here may represent a suitable tool for tracing the geographical origin of Italian Mozzarella.

Item Type:Article
ID Code:2425
Uncontrolled Keywords:Bacterial community, cheese, ingerprinting, food traceability, internal transcribed spacers-PCR, PDO authentication, phylogeny, 16S–23S spacer
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Publisher:The Society for Applied Microbiology
Copyright Holders:© 2006 The Authors, Journal compilation © 2006 The Society for Applied Microbiology
Deposited On:18 Aug 2009 10:07

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