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Interferon-gamma, tumor necrosis factor-alpha, and lipopolysaccharide promote chitotriosidase gene expression in human macrophages

Malaguarnera, Lucia and Musumeci, Maria and Di Rosa, Michelino and Scuto, Anna and Musumeci, Salvatore (2005) Interferon-gamma, tumor necrosis factor-alpha, and lipopolysaccharide promote chitotriosidase gene expression in human macrophages. Journal of Clinical Laboratory Analysis, Vol. 19 (3), p. 128-132. eISSN 1098-2825. Article.

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DOI: 10.1002/jcla.20063

Abstract

Human chitotriosidase (Chit), a chitinolytic enzyme, is a member of the chitinase family. In human plasma, Chit activity has been proposed as a biochemical marker of macrophage activation in several lysosomal diseases. Recently we found that Chit activity is higher in patients affected by Plasmodium falciparum malaria infection, suggesting that Chit may reflect induction of an immunological response. To assess this hypothesis, we evaluated the CHIT1 mRNA levels in human monocytes/macrophages (HMMs) following treatment with interferon-gamma (IFNϒ), tumor necrosis factor-alpha (TNFα), and lipopolysaccharide (LPS). Stimulation of macrophages with INF-ϒ, TNF-α, and LPS resulted in an increase in Chit activity as well as the levels of CHIT1 mRNA as measured by quantitative real-time PCR. The data presented in this article show that Chit plays a role in the response to the activation of INF-ϒ-, TNF-α-, and LPS-driven macrophages, suggesting that the production of Chit by macrophages could have biological relevance in the immune-response.

Item Type:Article
ID Code:2398
Status:Published
Refereed:Yes
Uncontrolled Keywords:Human monocyte/macrophages, chitotriosidase, cytokines, immune response
Subjects:Area 06 - Scienze mediche > MED/38 Pediatria generale e specialistica
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Wiley-Liss
eISSN:1098-2825
Deposited On:18 Aug 2009 10:07

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