HPLC–DAD determination of mepivacaine in cerebrospinal fluid from a fatal case: case report

Nieddu, Maria and Boatto, Gianpiero and Serra, Domenico and Soro, Aldo and Lorenzoni, Salvatore and Lubinu, Francesco (2007) HPLC–DAD determination of mepivacaine in cerebrospinal fluid from a fatal case: case report. Journal of Forensic Sciences, Vol. 52 (5), p. 1223-1224. eISSN 1556-4029. Article.

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DOI: 10.1111/j.1556-4029.2007.00526.x

Abstract

A fatal case involving mepivacaine-induced epidural anesthesia is described. The pathological findings were typical of cardiac shock from ischemic origin. Cerebrospinal fluid (CSF) was obtained several hours after death and mepivacaine was identified by gas chromatography-mass spectrometry (GC-MS). Its concentration was determined by high performance liquid chromatography with diode array detection (HPLC–DAD). Extraction from CSF was performed by deproteinization with acetonitrile. The mepivacaine concentration in the sample was 264 μg/mL. Concentrations of mepivacaine in CSF following epidural anesthesia are not reported in literature to our knowledge. This is the first reported case of death in which the mepivacaine concentration in CSF has been determined.

Item Type:	Article
ID Code:	2368
Status:	Published
Refereed:	Yes
Uncontrolled Keywords:	Forensic science, toxicology, mepivacaine hydrochloride, epidural anaesthesia, cerebrospinal fluid
Subjects:	Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:	002 Altri enti e centri di ricerca del Nord Sardegna > Azienda ASL1, Sassari > Unità operativa di patologia forense 001 Università di Sassari > 01 Dipartimenti > Farmaco, chimico, tossicologico
Publisher:	Blackwell / Wiley
eISSN:	1556-4029
Copyright Holders:	© 2007 American Academy of Forensic Sciences
Deposited On:	18 Aug 2009 10:07

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