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Relations between relative mRNA abundance and developmental competence of ovine oocytes

Leoni, Giovanni Giuseppe and Bebbere, Daniela and Succu, Sara and Berlinguer, Fiammetta and Mossa, Francesca and Galioto, M. and Bogliolo, Luisa and Ledda, Sergio and Naitana, Salvatore (2007) Relations between relative mRNA abundance and developmental competence of ovine oocytes. Molecular Reproduction and Development, Vol. 74 (2), p. 249-257. eISSN 1098-2795. Article.

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DOI: 10.1002/mrd.20442

Abstract

The present study was conducted to investigate the relation between in vitro developmental competence and the expression of a panel of developmentally important genes in germinal vesicle (GV) stage oocytes. One-month-old prepubertal and adult sheep oocytes were used as models of low and high quality gametes, respectively. Cumulus-oocyte complexes (COCs) derived from lambs and ewes were in vitro matured and fertilized, and their cleavage rate at 22, 26, and 32 hr post fertilization and the blastocyst yield were observed to assess their developmental potential. In parallel, the relative abundance (RA) of 11 genes was analyzed by semi-quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) assay in the two groups of oocytes. We observed similar maturation and fertilization rates in the two groups, but a significant lower rate of cleaved prepubertal oocytes (P < 0.05), a general delay in the timing of their first division (P < 0.01), and a lower blastocysts production (P < 0.05). The analysis of gene expression evidenced no difference in the RA of four transcripts [superoxide dismutase (SOD), ubiquitin, β-actin, cyclin B] in the two classes of oocytes, but a statistically lower RA of seven messenger RNAs (mRNA) [Na+K+ATPase, p34cdc2, Glucose-transporter I (Glut-1), Activin, Zona Occludens Protein 2 (PanZO2), Poli(A)Polymerase (PAP), E-Cadherin (E-Cad)] in the prepubertal oocytes compared to the adult ones. The present data show for the first time in the ovine species that the lower developmental competence is associated with deficiencies in the mRNAs storage during the oocyte growth.

Item Type:Article
ID Code:2365
Status:Published
Refereed:Yes
Uncontrolled Keywords:Gene expression, kinetic of development, oocyte quality
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Wiley-Liss
eISSN:1098-2795
Copyright Holders:© 2006 WILEY-LISS
Deposited On:18 Aug 2009 10:07

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