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Identification of a novel candidate gene, CASC2, in a region of common allelic loss at chromosome 10q26 in human endometrial cancer

Baldinu, Paola and Cossu, Antonio and Manca, Antonella and Satta, Maria Paola and Sini, Maria Cristina and Rozzo, Carla and Dessole, Salvatore and Cherchi, Pier Luigi and Gianfrancesco, Fernando and Pintus, Adriana and Carboni, Annangela and Deiana, Angelo and Tanda, Francesco and Palmieri, Giuseppe (2004) Identification of a novel candidate gene, CASC2, in a region of common allelic loss at chromosome 10q26 in human endometrial cancer. Human Mutation, Vol. 23 (4), p. 318-326. eISSN 1098-1004. Article.

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DOI: 10.1002/humu.20015

Abstract

Allelic deletions, which are suggestive for the presence of tumor suppressor genes, represent a common event in endometrial cancer (EC). Previous loss-of-heterozygosity studies for human chromosome 10q identified a candidate deletion interval at 10q25-q26, which we further narrowed to a 160-kb region at 10q26, bounded by markers D10S1236 and WIAF3299. Using a positional candidate approach, we identified three alternative transcripts of a novel human gene, CASC2 (cancer susceptibility candidate 2; formely C10orf5). One of such transcripts, CASC2a, encodes a short protein of 102 amino acids with no similarity to any other known gene product. Three (7%) CASC2a mutations were identified in tumor DNA from 44 EC patients. While c.-156G>T and c.22C>T (p.Pro8Ser) are sequence variants with unknown functional significance, c.84delA is a mutation with a truncation effect on the predicted protein (p. Asn28fsX50). Expression studies by real-time RT-PCR on several normal and tumor cells revealed that CASC2a mRNA is downregulated in cancer, suggesting that it may act as a potential tumor suppressor gene. The very low mutation rate seems to also indicate that inactivation of CASC2a might probably be due to mechanisms different from genetic alterations

Item Type:Article
ID Code:2304
Status:Published
Refereed:Yes
Uncontrolled Keywords:Endometrial cancer, EC, allelic deletion, positional mapping, cancer, mutation analysis, mRNA expression analysis; CASC2
Subjects:Area 06 - Scienze mediche > MED/40 Ginecologia e ostetricia
Area 06 - Scienze mediche > MED/08 Anatomia patologica
Divisions:001 Università di Sassari > 03 Istituti > Anatomia patologica
001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di chimica biomolecolare, Sassari
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di genetica delle popolazioni, Alghero
Publisher:Wiley-Liss
eISSN:1098-1004
Deposited On:18 Aug 2009 10:07

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