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DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification

Isachenko, Eugenia and Isachenko, Vladimir and Katkov, Igor I. and Rahimi, G. and Schöndorf, T. and Mallmann, P. and Dessole, Salvatore and Nawroht, Frank (2004) DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification. Human Reproduction, Vol. 19 (4), p. 932-939. eISSN 1460-2350. Article.

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DOI: 10.1093/humrep/deh194

Abstract

BACKGROUND: In contrast to the technique of conventional freezing, the vitrification of spermatozoa requires high cooling rates (720 000°K/min), which could be damaging for spermatozoa. The aim of our study was to compare slowly frozen and vitrified spermatozoa in terms of their post-thaw DNA integrity and motility. METHODS: Semen samples were prepared according to the routine swim-up technique and divided into aliquots for comparison of fresh, conventionally frozen and vitrified spermatozoa from the same ejaculate in the presence or absence of cryoprotectants. Spermatozoa motility and DNA integrity were determined. RESULTS: The motility of spermatozoa conventionally (slowly) frozen with a cryoprotectant was similar to that recorded for spermatozoa vitrified in the absence of cryoprotectant (47 versus 52%). The DNA integrity was unaffected by the cryopreservation method or presence of cryoprotectants. CONCLUSION: The vitrification of human spermatozoa in the absence of conventional cryoprotectants is indeed feasible. The DNA integrity of vitrified sperm is comparable with that shown by standard slow-frozen/thawed spermatozoa, yet the method is quick and simple and does not require special cryobiological equipment.

Item Type:Article
ID Code:2297
Status:Published
Refereed:Yes
Uncontrolled Keywords:Comet assay, cryopreservation, human, sperm DNA, vitrification
Subjects:Area 06 - Scienze mediche > MED/40 Ginecologia e ostetricia
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Oxford University Press
eISSN:1460-2350
Copyright Holders:© European Society of Human Reproduction and Embryology 2004
Deposited On:18 Aug 2009 10:07

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