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Variations in the molecular and physiological characteristics and the virulence of Monilinia fructicola, M. fructigena and M. laxa isolates

Pizzuolo, Pablo Humberto and Chilosi, Gabriele and Balmas, Virgilio and Aleandri, Maria Pia and Vitale, Salvatore and Luongo, Laura and Corazza, Luciana and Magro, Paolo (2006) Variations in the molecular and physiological characteristics and the virulence of Monilinia fructicola, M. fructigena and M. laxa isolates. Phytopathologia Mediterranea, Vol. 45 , p. 139-152. eISSN 1593-2095. Article.

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DOI: 10.1400/54737

Abstract

Twenty-three Monilinia isolates of various origin were identified and compared by morphological means and by biomolecular protocols based on random amplified polymorphic DNA (RAPD) analysis, and on PCR detection by species-specific primer pairs. The identification of most Monilinia isolates by PCR and RAPD confirmed the identification by morphological criteria, although two isolates initially identified as M. fructigena by morphological means were attributed to M. fructicola by the biomolecular techniques. Monilinia isolates were also compared in terms of their in vitro pectolytic activity and isoenzyme patterns. All the isolates produced polygalacturonase and pectin methylesterase, but pectin lyase activity was found only in M. fructicola and M. fructigena, and not in most M. laxa isolates. The Monilinia isolates differed significantly in their isoenzyme patterns. Based on RAPD-PCR and PCR amplification and pectolytic isoenzymes, the isolates analysed clustered in three major groups, each corresponding to a Monilinia species. Artificial inoculations in peach and pear fruits revealed differences in virulence between the Monilinia species and within isolates. No correlation was found between the amount of pectolytic enzymes produced in vitro and virulence. Variations between and within the Monilinia species may depend at the physiological level on variations in the isoenzyme patterns of the pectolytic enzymes.

Item Type:Article
ID Code:2249
Status:Published
Refereed:Yes
Uncontrolled Keywords:Brown rot, pectin lyase, PCR-RAPD, pectin methylesterase, polygalacturonase
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Unione Fitopatologica Mediterranea
eISSN:1593-2095
Deposited On:18 Aug 2009 10:07

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