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Molecular analysis of "Fomitiporia mediterranea" isolates from esca-affected grapevines in Southern Italy

Ciccarone, Claudio and Graniti, Antonio and Schiaffino, Angela and Marras, Francesco Salvatore (2004) Molecular analysis of "Fomitiporia mediterranea" isolates from esca-affected grapevines in Southern Italy. Phytopathologia Mediterranea , p. 268-272. ISSN 1593-2095. Article.

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DOI: 10.1400/14597

Abstract

Four Fomitiporia isolates representative of a collection of about 300 isolates from esca-affected grapevines in southern Italy (Apulia, Campania and Abruzzo) were examined by molecular methods. The DNA of each isolate was analysed by means of polymerase chain reaction (PCR amplification) using ITS5 and ITS4 primers. An amplification product of 740 bp was obtained from all isolates. The denaturated products had the same migration pattern when analysed by single-strand conformation polymorphism. The PCR fragments that included the ribosomal ITS1-5.8S-ITS2 region were sequenced. In order to ascertain the taxonomic identity of the isolates, the ITS sequences were compared with those of Fomitiporia mediterranea, F. punctata and F. robusta deposited in the GenBank. The ITS sequences of the isolates were uniform and homologous with those of the type culture of Fomitiporia mediterranea M. Fischer, to which the southern Italian isolates were compared.

Item Type:Article
ID Code:2244
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fomitiporia punctata, Fomitiporia robusta, ITS sequences, wood decay
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Unione Fitopatologica Mediterranea
ISSN:1593-2095
Deposited On:18 Aug 2009 10:07

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