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The Strange case of a biofilm-forming strain of Pichia fermentans, which controls Monilinia brown rot on apple but is pathogenic on peach fruit

Giobbe, Sara and Marceddu, Salvatore and Scherm, Barbara and Zara, Giacomo and Mazzarello, Vittorio Lorenzo Giuseppe and Budroni, Marilena and Migheli, Quirico (2007) The Strange case of a biofilm-forming strain of Pichia fermentans, which controls Monilinia brown rot on apple but is pathogenic on peach fruit. FEMS Yeast Research, Vol. 7 (8), p. 1389-1398. eISSN 1567-1356. Article.

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DOI: 10.1111/j.1567-1364.2007.00301.x

Abstract

A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the apple fruit surface, this yeast formed a thin biofilm but failed to colonize the underlying tissues. When inoculated into wounds artificially inflicted to peach fruit or when sprayed onto the surface of peach fruit, the same strain showed an unexpected pathogenic behaviour, causing rapid decay of fruit tissues even in the absence of M. fructicola. Both optical and scanning electron microscopy were used to evaluate the pattern of fruit tissue colonization by P. fermentans. While on apple surface and within the apple wound the antagonist retained its yeast-like shape, colonization of peach fruit tissue was always characterized by a transition from budding growth to pseudohyphal growth. These results suggest that pseudohyphal growth plays a major role in governing the potential pathogenicity of P. fermentans, further emphasizing the importance of a thorough risk assessment for the safe use of any novel biocontrol agent.

Item Type:Article
ID Code:213
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fruit decay, Candida lambica, biological control, risk assessment, biohazard, dimorphic transition
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Area 05 - Scienze biologiche > BIO/16 Anatomia umana
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di scienze delle produzioni alimentari, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Blackwell / Wiley
eISSN:1567-1356
Copyright Holders:© 2007 Federation of European Microbiological Societies
Deposited On:18 Aug 2009 10:01

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