titoli, abstracts, parole chiave >>>
Differential lectin binding patterns in the oviductal ampulla of the horse during oestrus

Desantis, Salvatore and Ventriglia, Gianluca and Zubani, Dolores and Corriero, Aldo and Deflorio, Michele and Acone, Franca and Palmieri, Giovanni and De Metrio, Gregorio (2005) Differential lectin binding patterns in the oviductal ampulla of the horse during oestrus. European Journal of Histochemistry, Vol. 49 (2), p. 139-149. ISSN 1121-760X. Article.

[img]
Preview
Full text disponibile come PDF Richiede visualizzatore di PDF come GSview, Xpdf o Adobe Acrobat Reader
Available under License Creative Commons Attribution.

243Kb

Alternative URLs:

Abstract

We investigated the oligosaccharide sequence of glycoconjugates, mainly sialoglycoconjugates, in the horse oviductal ampulla during oestrus by means of lectin and pre-lectin methods such as the KOH-neuraminidase procedure to remove sialic acid residues and incubation with N-glycosidase F to cleave N-linked glycans. Ciliated cells displayed N-linked oligosaccharides throughout the cytoplasm. The cilia glycocalyx expressed both N- and O-linked (mucin-type) oligosaccharides, both showing a high variety of terminal sequences. In the most non-ciliated cells, the whole cytoplasm contained N-linked oligosaccharides with terminal αGal as well as mucin-type glycans with terminal Forssman pentasaccharides. In a few scattered non-ciliated cells, the whole cytoplasm displayed sialylated N-linked oligosaccharides with terminal Neu5Ac-GalNAc and O-linked glycans terminating with neutral and/or αGalNAc, Neu5Acα2,6Gal/ GalNAc, Neu5AcGalβ1,3GalNAc. Supra-nuclear granules, probably Golgi zones, of non-ciliated cells showed mainly O-linked glycans rich in sialic acid residues. The luminal surface of non-ciliated cells showed N-linked oligosaccharides, containing terminal/internal αMan/αGlc, βGlcNAc and terminal αGal, as well as mucin-type oligosaccharides terminating with a large variety of either neutral saccharides or sialylated sequences. Apical protrusions containing O-linked oligosaccharides with terminal Forssman pentasaccharide, Neu5Ac-Galβ1,4GlcNAc, Neu5Ac-GalNAc were seen in nonciliated cells scattered along the epithelium. These findings show the presence of sialoglycoconjugates in the oviductal ampulla epithelium of the mare and the existence of different lectin binding profiles between ciliated and non-ciliated (secretory) cells, as well as the presence of non-ciliated cell sub-types which might determine functional differences along the ampullary epithelium of mare oviduct.

Item Type:Article
ID Code:210
Status:Published
Refereed:Yes
Uncontrolled Keywords:Histochemistry, lectins, sialic acid, glycoconjugates, oviduct, horse
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Luigi Ponzio
ISSN:1121-760X
Copyright Holders:© 2005 European Journal of Histochemistry, © 2009 PagePress
Deposited On:18 Aug 2009 10:01

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page