Sotgia, Salvatore and Carru, Ciriaco and Franconi, Flavia and Fiori, Paolo Battista and Manca, Salvatorica and Pettinato, Silvestro and Magliona, Stefania and Ginanneschi, Renzo and Deiana, Luca and Zinellu, Angelo (2008) Rapid quantification of total genomic DNA methylation degree by short-end injection capillary zone electrophoresis. Journal of Chromatography A, Vol. 1185 (1), p. 145-150. ISSN 0021-9673. Article.
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We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation.
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