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Rapid quantification of total genomic DNA methylation degree by short-end injection capillary zone electrophoresis

Sotgia, Salvatore and Carru, Ciriaco and Franconi, Flavia and Fiori, Paolo Battista and Manca, Salvatorica and Pettinato, Silvestro and Magliona, Stefania and Ginanneschi, Renzo and Deiana, Luca and Zinellu, Angelo (2008) Rapid quantification of total genomic DNA methylation degree by short-end injection capillary zone electrophoresis. Journal of Chromatography A, Vol. 1185 (1), p. 145-150. ISSN 0021-9673. Article.

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DOI: 10.1016/j.chroma.2008.01.032

Abstract

We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation.

Item Type:Article
ID Code:2019
Status:Published
Refereed:Yes
Uncontrolled Keywords:DNA methylation, methylcytosine, short-end injection, capillary electrophoresis
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
001 Università di Sassari > 01 Dipartimenti > Farmaco, chimico, tossicologico
001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
002 Altri enti e centri di ricerca del Nord Sardegna > Azienda ASL1, Sassari > Laboratorio generale di base
Publisher:Elsevier B. V.
ISSN:0021-9673
Deposited On:18 Aug 2009 10:06

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