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Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA

Alberti, Alberto and Robino, Patrizia and Chessa, Bernardo and Rosati, Sergio and Addis, Maria Filippa and Mercier, Pascale and Mannelli, Alessandro and Cubeddu, Tiziana and Profiti, Margherita and Bandino, Ennio and Thiery, Richard and Pittau, Marco (2008) Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA. Veterinary Microbiology, Vol. 128 (1-2), p. 81-89. ISSN 0378-1135. Article.

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DOI: 10.1016/j.vetmic.2007.09.020

Abstract

This paper reports the identification and characterisation of a 60 kDa surface protein antigen (P60) of Mycoplasma capricolum subspecies capricolum (Mcc), and describes its diagnostic application. Genomic localization and presence in P60 of conserved functional domains suggested a structural and functional relationship with the immunodominant antigen P48 of Mycoplasma agalactiae, a basic membrane protein. A rP60-ELISA was developed, and it resulted in a high specificity for Mcc infections after evaluation with 125 goat sera. The comparison with an existent ELISA based on whole Mcc cell lysates showed that the two assays have comparable sensitivities, but the rP60-ELISA has the significant advantage of a greater specificity. Results indicate that P60 is a potential marker of Mcc infection, especially useful in areas where the presence of M. capricolum subspecies capripenumoniae is also reported.

Item Type:Article
ID Code:2000
Status:Published
Refereed:Yes
Uncontrolled Keywords:Mycoplasma capricolum subspecies capricolum, contagious agalactia, ELISA, basic membrane proteins, P60
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/05 Malattie infettive degli animali domestici
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari > Sezione diagnostica, Nuoro
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
Publisher:Elsevier
ISSN:0378-1135
Deposited On:18 Aug 2009 10:06

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