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Structural perturbation of alphaB-crystallin by zinc and temperature related to its chaperone-like activity

Coi, Alessio and Bianucci, Anna Maria and Bonomi, Francesco and Rasmussen, Patrizia and Mura, Giovanni Maria and Ganadu, Maria Luisa Margherita (2008) Structural perturbation of alphaB-crystallin by zinc and temperature related to its chaperone-like activity. International Journal of Biological Macromolecules, Vol. 42 (3), p. 229-234. ISSN 0141-8130. Article.

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DOI: 10.1016/j.ijbiomac.2007.10.012


αB-crystallin is a small heat shock protein that shows chaperone-like activity, as it protects the aggregation of denatured proteins. In this work, the possible relationships between structural characteristics and the biological activity of αB-crystallin were investigated on the native protein and on the protein undergoing the separate effects of metal ligation and temperature. The chaperone-like activity of αB-crystallin increased in the presence of zinc and when temperature was increased. By using fluorescent probes to monitor hydrophobic surfaces on αB-crystallin, it was found that exposed hydrophobic patches on the protein surface increased significantly both in the presence of zinc and when the temperature was raised from 25 to 37°C. The zinc-induced increased exposure of lipophilic residues is in agreement with theoretical calculations performed on 3D-models of monomeric αB-crystallin, and may be significant to its increased biological activity.

Item Type:Article
ID Code:1995
Uncontrolled Keywords:αB-crystallin, chaperone-like activity, fluorimetric measurements, structural perturbation
Subjects:Area 03 - Scienze chimiche > CHIM/12 Chimica dell'ambiente e dei beni culturali
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Copyright Holders:© 2007 Elsevier B.V.
Deposited On:18 Aug 2009 10:06

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