Deriu, Antonella and Zanetti, Stefania Anna Lucia and Sechi, Leonardo Antonio and Marongiu, Bruno and Piras, Alessandra and Porcedda, Silvia and Tuveri, Enrica (2008) Antimicrobial activity of Inula helenium L. essential oil against Gram-positive and Gram-negative bacteria and Candida spp. International Journal of Antimicrobial Agents, Vol. 31 (6), p. 588-590. ISSN 0924-8579. Article.
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In this work we report results regarding the in vitro antimicrobial activity of Inula helenium L. dried root extracts obtained by stepwise supercritical fluid extraction (SFE) and by hydrodistillation (HD). Inula helenium L. (Compositae family) is a perennial plant widely occurring in Europe and East Asia. Its oil is one of the richest sources of sesquiterpenoid lactones, which have strong anthelminthic activity and have the potential to induce detoxifying enzymes. Inula helenium L. roots (C-040705130905) were purchased from Minardi (Bagnacavallo-Ravenna, Italy). Supercritical CO2 extraction was performed in a laboratory apparatus equipped with a 400 cm3 extraction vessel operated in the single-pass mode of passing CO2 through the fixed bed of charged vegetable particles. Extraction was carried out in a semibatch mode: batch charging of vegetable matter and continuous flow solvent. HD was performed for 4 h in a circulatory Clevenger-type apparatus up to exhaustion of the oil contained in the matrix, which was the same material as used in the SFE.
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