Madeddu, Paolo Roberto and Varoni, Maria Vittoria and Demontis, Maria Piera and Fattaccio, Maria Caterina and Pinna Parpaglia, Paolo and Glorioso, Nicola (1994) A Kallikrein-like enzyme in the aorta of normotensive and hypertensive rats. Hypertension, Vol. 23 (6), p. 899-902. eISSN 1524-4563. Article.
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We evaluated whether vascular kallikrein is altered in rats with genetic or experimental hypertension. Group 1 was infused intraperitoneally with angiotensin II (Ang II) or vehicle for 4 weeks; group 2 was injected subcutaneously with deoxycorticosterone (75 mumol/kg once a week) or vehicle for 4 weeks; group 3 consisted of uninephrectomized rats on high sodium intake given deoxycorticosterone or vehicle; and group 4 consisted of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. Active and total kallikrein activity was measured in abdominal aortic homogenates using an amidolytic assay. Ang II increased systolic blood pressure at a dose of 400 nmol/kg per day (146 +/- 6 versus 123 +/- 3 mm Hg in controls, P < .01) but not at 80 nmol/kg per day. Deoxycorticosterone did not increase blood pressure except in uninephrectomized rats on high salt (173 +/- 6 versus 135 +/- 4 mm Hg in controls, P < .01). Blood pressure averaged 194 +/- 2 mm Hg in SHR and 123 +/- 3 mm Hg in WKY rats. Vascular kallikrein was similar in rats given Ang II or vehicle. In deoxycorticosterone-treated rats total kallikrein was higher than in controls (9.2 +/- 0.8 versus 3.5 +/- 0.1 pkat/mg protein, P < .05), whereas active kallikrein did not differ (0.09 +/- 0.04 versus 0.09 +/- 0.03 pkat/mg protein, P = NS). A similar pattern was observed in uninephrectomized deoxycorticosterone-treated rats (active, 0.09 +/- 0.03 versus 0.10 +/- 0.04, P = NS; total, 7.4 +/- 0.7 versus 4.1 +/- 0.2 pkat/mg protein, P < .05). Kallikrein activity was higher in SHR compared with WKY rats (active, 0.34±0.04 versus 0.10±0.03; total, 9.5 + 1.2 versus 4.6±0.3 pkat/mg protein, P<.05). In conclusion, a kallikrein-like enzyme is present in rat aorta. The activity of this enzyme is elevated in rats with genetic hypertension, and it may be regulated by mineralocorticoids.
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