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A Kallikrein-like enzyme in the aorta of normotensive and hypertensive rats

Madeddu, Paolo Roberto and Varoni, Maria Vittoria and Demontis, Maria Piera and Fattaccio, Maria Caterina and Pinna Parpaglia, Paolo and Glorioso, Nicola (1994) A Kallikrein-like enzyme in the aorta of normotensive and hypertensive rats. Hypertension, Vol. 23 (6), p. 899-902. eISSN 1524-4563. Article.

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We evaluated whether vascular kallikrein is altered in rats with genetic or experimental hypertension. Group 1 was infused intraperitoneally with angiotensin II (Ang II) or vehicle for 4 weeks; group 2 was injected subcutaneously with deoxycorticosterone (75 mumol/kg once a week) or vehicle for 4 weeks; group 3 consisted of uninephrectomized rats on high sodium intake given deoxycorticosterone or vehicle; and group 4 consisted of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. Active and total kallikrein activity was measured in abdominal aortic homogenates using an amidolytic assay. Ang II increased systolic blood pressure at a dose of 400 nmol/kg per day (146 +/- 6 versus 123 +/- 3 mm Hg in controls, P < .01) but not at 80 nmol/kg per day. Deoxycorticosterone did not increase blood pressure except in uninephrectomized rats on high salt (173 +/- 6 versus 135 +/- 4 mm Hg in controls, P < .01). Blood pressure averaged 194 +/- 2 mm Hg in SHR and 123 +/- 3 mm Hg in WKY rats. Vascular kallikrein was similar in rats given Ang II or vehicle. In deoxycorticosterone-treated rats total kallikrein was higher than in controls (9.2 +/- 0.8 versus 3.5 +/- 0.1 pkat/mg protein, P < .05), whereas active kallikrein did not differ (0.09 +/- 0.04 versus 0.09 +/- 0.03 pkat/mg protein, P = NS). A similar pattern was observed in uninephrectomized deoxycorticosterone-treated rats (active, 0.09 +/- 0.03 versus 0.10 +/- 0.04, P = NS; total, 7.4 +/- 0.7 versus 4.1 +/- 0.2 pkat/mg protein, P < .05). Kallikrein activity was higher in SHR compared with WKY rats (active, 0.34±0.04 versus 0.10±0.03; total, 9.5 + 1.2 versus 4.6±0.3 pkat/mg protein, P<.05). In conclusion, a kallikrein-like enzyme is present in rat aorta. The activity of this enzyme is elevated in rats with genetic hypertension, and it may be regulated by mineralocorticoids.

Item Type:Article
ID Code:194
Uncontrolled Keywords:Blood pressure, bradykinin, angiotensins, mineralocorticoids
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/07 Farmacologia e tossicologia veterinaria
Area 06 - Scienze mediche > MED/09 Medicina interna
Divisions:001 Università di Sassari > 03 Istituti > Clinica medica generale e terapia medica
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Lippincott Williams & Wilkins
Copyright Holders:© 1994 American Heart Association
Deposited On:18 Aug 2009 10:01

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