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A Proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries

Lepedda, Antonio Junior and Cigliano, Antonio and Cherchi, Gian Mario and Spirito, Rita and Maggioni, Marco and Carta, Franco and Turrini, Francesco Michelangelo and Edelstein, Celina and Scanu, Angelo M. and Formato, Marilena (2009) A Proteomic approach to differentiate histologically classified stable and unstable plaques from human carotid arteries. Atherosclerosis, Vol. 203 (1), p. 112-118. ISSN 0021-9150. Article.

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DOI: 10.1016/j.atherosclerosis.2008.07.001

Abstract

Objectives. By using proteomics we isolated and identified proteins that were expressed/retained in stable and unstable human carotid artery atherosclerotic plaques.
Methods. The criteria for plaque instability were the presence of a thin fibrous cap or fissured cap covering the foamy or necrotic core, and the presence of overt, hemorrhagic, ulcerated or thrombotic plaques. Proteins were extracted from finely minced endarterectomy specimens (19 stable and 29 unstable plaques) and separated by two-dimensional gel electrophoresis. Coomassie Blue-stained gels were analysed using PD-Quest software.
Results. A total of 57 distinct spots corresponding to 33 different proteins were identified by matrix assisted laser desorption/ionization mass spectrometry using the NCBI database. Most of the spots were present in both types of extracts, although significantly (p < 0.05) differing in abundance between them. Compared to stable plaque, unstable ones showed reduced abundance of: protective enzymes SOD3 and GST, small heat shock proteins HSP27 and HSP20, annexin A10, and Rho GDI. In unstable plaques the more abundant proteins were: ferritin light subunit, SOD 2 and fibrinogen fragment D. For fibrinogen fragment D, the increased levels in unstable versus stable plaques was confirmed by Western blot analysis.
Conclusions. Since many of the differentially expressed proteins are known to have a functional role in inflammation and oxidative stress, we speculate that they may be involved in events relating to plaque stability.

Item Type:Article
ID Code:1919
Status:Published
Refereed:Yes
Uncontrolled Keywords:Atherosclerosis, proteomics, stable and unstable carotid plaque
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Nurex Bioresearch Sassari, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
Publisher:Elsevier Ireland
ISSN:0021-9150
Deposited On:18 Aug 2009 10:06

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