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Vaccination with recombinant oncosphere antigens reduces the susceptibility of sheep to infection with Taenia multiceps

Gauci, Charles G. and Vural, Gülay and Oncel, Taraneh and Varcasia, Antonio and Damian, Veronica and Kyngdon, Craig T. and Craig, Philip S. and Anderson, Garry A. and Lightowlers, Marshall W. (2008) Vaccination with recombinant oncosphere antigens reduces the susceptibility of sheep to infection with Taenia multiceps. International Journal for Parasitology, Vol. 38 (8-9), p. 1041-1050. ISSN 0020-7519. Article.

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DOI: 10.1016/j.ijpara.2007.11.006

Abstract

Taenia multiceps is a cestode parasite, the larval stage of which encysts in the brain of sheep, goats and cattle causing an often fatal condition. The parasite also causes zoonotic infections in humans. Homologues of the recombinant oncosphere vaccine antigens from Taenia ovis and other Taenia species were identified in T. multiceps. Sequencing of the associated T. multiceps genes and cloning of the encoding mRNA has revealed conserved features in the genes and proteins. The T. multiceps oncosphere proteins, designated Tm16 and Tm18, contain a predicted secretory signal and fibronectin type III domain. The recombinant Tm16 and Tm18 proteins were successfully expressed in Escherichia coli as fusion proteins with GST. The antigens, formulated with Quil A adjuvant, were tested in a vaccine trial in sheep. The antigens stimulated immunity in sheep against challenge infection with T. multiceps eggs. Five of nine control sheep died due to a challenge infection with T. multiceps whereas none of 20 vaccinated animals died as a result of the parasite challenge (P = 0.001). In addition, vaccination with the Tm16 protein, or Tm16 plus Tm18, induced significant protection against the number of parasites encysting in the brain as a result of the challenge infection (P = 0.023, P = 0.015, respectively). No clear relationship was apparent between the level of specific serum antibody in vaccinated animals and either the presence or absence of parasites or the number of parasites that occurred in some of the vaccinated animals. We believe this study is the first description of recombinant vaccine-related investigations for T. multiceps. The recombinant oncosphere antigens identified may allow development of effective vaccination strategies against T. multiceps infection in sheep. They raise the potential for the development of a combined vaccine with the Echinococcus granulosus EG95 antigen for prevention of T. multiceps as well as preventing the transmission of cystic hydatid disease.

Item Type:Article
ID Code:1827
Status:Published
Refereed:Yes
Uncontrolled Keywords:Taenia multiceps, sheep vaccine, oncosphere, antigens, vaccination, recombinant
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/06 Parassitologia e malattie parassitarie degli animali
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Elsevier
ISSN:0020-7519
Copyright Holders:© 2007 Australian Society for Parasitology
Additional Information:Pubblicato online il 24 novembre 2007.
Deposited On:18 Aug 2009 10:06

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