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Knockdown of cytosolic 5'-nucleotidase II (cN-II) reveals that its activity is essential for survival in astrocytoma cells

Careddu, Maria Giovanna and Allegrini, Simone and Pesi, Rossana and Camici, Marcella and Garcia-Gil, Mercedes and Tozzi, Maria Grazia (2008) Knockdown of cytosolic 5'-nucleotidase II (cN-II) reveals that its activity is essential for survival in astrocytoma cells. Biochimica et Biophysica Acta. Molecular Cell Research, Vol. 1783 (8), p. 1529-1535. ISSN 0167-4889. Article.

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DOI: 10.1016/j.bbamcr.2008.03.018

Abstract

IMP preferring cytosolic 5'-nucleotidase (cN-II) is an ubiquitous nucleotide hydrolysing enzyme. The enzyme is widely distributed and its amino acid sequence is highly conserved among vertebrates. Fluctuations of cN-II activity have been associated with the pathogenesis of neurological disorders. The enzyme appears to be involved in the regulation of the intracellular availability of the purine precursor IMP and also of GMP and AMP, but the contribution of this activity and of its regulation to cell metabolism and to CNS cell functions remains uncertain. To address this issue, we used a vector based short hairpin RNA (shRNA) strategy to knockdown cN-II activity in human astrocytoma cells. Our results demonstrated that 53 h after transduction, cN-II mRNA was reduced to 17.9 ± 0.03% of control cells. 19 h later enzyme activity was decreased from 0.7 ± 0.026 mU/mg in control ADF cells to 0.45 ± 0.046 mU/mg, while cell viability (evaluated by the MTT reduction assay) decreased up to 0.59 ± 0.01 (fold vs control) and caspase 3 activity increased from 136 ± 5.8 pmol min-1 mg-1 in control cells to 639 ± 37.5 pmol min-1 mg-1 in silenced cells, thus demonstrating that cN-II is essential for cell survival. The decrease of enzyme activity causes apoptosis of the cultured cells without altering intracellular nucleotide and nucleoside concentration or energy charge. Since cN-II is highly expressed in tumour cells, our finding offers a new possible therapeutical approach especially against primary brain tumours such as glioblastoma, and to ameliorate chemotherapy against leukemia.

Item Type:Article
ID Code:1809
Status:Published
Refereed:Yes
Uncontrolled Keywords:cN-II, cytosolic 5'-nucleotidase, purine analogs, shRNA, RNAi, apoptosis, ADF
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze del farmaco
Publisher:Elsevier Science
ISSN:0167-4889
Deposited On:18 Aug 2009 10:06

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