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Correlation between cell lipid content, gene expression and fermentative behaviour of two Saccharomyces cerevisiae wine strains

Zara, Giacomo and Bardi, Luca and Belviso, Simona and Farris, Giovanni Antonio and Zara, Severino and Budroni, Marilena (2008) Correlation between cell lipid content, gene expression and fermentative behaviour of two Saccharomyces cerevisiae wine strains. Journal of Applied Microbiology, Vol. 104 (3), p. 906-914. eISSN 1365-2672. Article.

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DOI: 10.1111/j.1365-2672.2007.03608.x

Abstract

Aim: To verify a possible correlation between cell lipid composition, expression of key genes in lipid metabolism and fermentative behaviour of Saccharomyces cerevisiae wine strains. Methods and Results: The fermentative abilities of two commercial wine strains of S. cerevisiae were tested under stressful conditions. Cell number, glucose and fructose concentrations, expression of ACS1, ACS2, ACC1, OLE1, ERG9, ERG10, ARE1 and ARE2 and lipid content were evaluated. The strain that failed to complete the fermentation had lower amounts of C16:1 and C16:0 fatty acids at the beginning of fermentation (0 h) and late logarithmic phase (72 h). While the amount of C18:1 in this strain was lower than that in the strain that completed the fermentation at 0 h, same levels were observed for both strains at 72 h. The sterol levels were generally higher in the strain that failed to complete the fermentation. Gene expression generally increased from the beginning of the fermentation to the late logarithmic phase in both strains. Conclusion: A positive correlation between good fermentative ability, elevated fatty acid content and ACC1 gene expression has been identified. Significance and Impact of the Study: The cell lipid content at the time of inoculum and expression of ACC1 gene of starter strains should be carefully considered in order to identify the possible stuck/sluggish fermentations.

Item Type:Article
ID Code:1800
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fermentative behaviour, gene transcription, lipids, quantitative, RT-PCR, Saccharomyces cerevisiae
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:The Society for Applied Microbiology
eISSN:1365-2672
Additional Information:Pubblicato online il 25 ottobre 2007.
Deposited On:18 Aug 2009 10:06

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