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Exploitation of the semi-homothallic life cycle of Saccharomyces cerevisiae for the development of breeding strategies

Zara, Giacomo and Mannazzu, Ilaria Maria and Sanna, Maria Lina and Orro, Davide and Farris, Giovanni Antonio and Budroni, Marilena (2008) Exploitation of the semi-homothallic life cycle of Saccharomyces cerevisiae for the development of breeding strategies. FEMS Yeast Research, Vol. 8 (7), p. 1147-1154. eISSN 1567-1364. Article.

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DOI: 10.1111/j.1567-1364.2008.00393.x


A strain of Saccharomyces cerevisiae having desirable winemaking properties and high spore viability was bred from a semi-homothallic parent strain with similar winemaking properties but that produced sixfold fewer viable spores. Because the parent was homozygous for HO and for the MATa allele at both silent HMR and HML loci, it produced two MATa and two nonmating progeny per ascus. To obtain a segregant able to mate with the stable MATa progeny, a strain of the nonmating progeny, previously subjected to HO distruption with a KanMX4 cassette, was used. The resultant MATαho::KanMX4 transformant was mated to a MATa HO segregant and the diploid produced was sporulated to allow the isolation of a semi-homothallic diploid segregant designated 2D that lacked the KanMX4-disrupted HO allele as confirmed by sequence analysis. Genetic analysis indicated greater homozygosity in 2D than in the parent as assessed by PCR at five loci. The sugar consumption profiles of both 2D and the parent in grape juice fermentations were the same. Acetaldehyde levels and postfermentation biofilm formation were higher in 2D than in the parent. Because 2D has acceptable winemaking characteristics but produces significantly more viable spores than the parent strain, it will be useful in future breeding efforts.

Item Type:Article
ID Code:1770
Uncontrolled Keywords:Saccharomyces cerevisiae, flor, biofilm, semi-homothallic life cycle, genetic stability, wine strains
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:Blackwell / Wiley
Copyright Holders:© 2008 Federation of European Microbiological Societies
Additional Information:Pubblicato online il 10 giugno 2008.
Deposited On:18 Aug 2009 10:06

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