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Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes

Muresu, Rosella and Polone, Elisa and Sulas, Leonardo and Baldan, Barbara and Tondello, Alessandra and Delogu, Giuseppe and Cappuccinelli, Pietro Antonio and Alberghini, Sara and Benhizia, Yacine and Benhizia, Hayet and Benguedouar, Ammar and Mori, Bruno and Calamassi, Roberto and Dazzo, Frank B. and Squartini, Andrea (2008) Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes. FEMS Microbiology Ecology, Vol. 63 (3), p. 383-400. eISSN 1574-6941. Article.

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DOI: 10.1111/j.1574-6941.2007.00424.x

Abstract

A previous analysis showed that Gammaproteobacteria could be the sole recoverable bacteria from surface-sterilized nodules of three wild species of Hedysarum. In this study we extended the analysis to eight Mediterranean native, uninoculated legumes never previously investigated regarding their root-nodule microsymbionts. The structural organization of the nodules was studied by light and electron microscopy, and their bacterial occupants were assessed by combined cultural and molecular approaches. On examination of 100 field-collected nodules, culturable isolates of rhizobia were hardly ever found, whereas over 24 other bacterial taxa were isolated from nodules. None of these nonrhizobial isolates could nodulate the original host when reinoculated in gnotobiotic culture. Despite the inability to culture rhizobial endosymbionts from within the nodules using standard culture media, a direct 16S rRNA gene PCR analysis revealed that most of these nodules contained rhizobia as the predominant population. The presence of nodular endophytes colocalized with rhizobia was verified by immunofluorescence microscopy of nodule sections using an Enterobacter-specific antibody. Hypotheses to explain the nonculturability of rhizobia are presented, and pertinent literature on legume endophytes is discussed.

Item Type:Article
ID Code:1723
Status:Published
Refereed:Yes
Uncontrolled Keywords:Rhizobia, endophytes, wild legumes, nonculturable
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto per il sistema produzione animale in ambiente Mediterraneo, Sassari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Blackwell / Wiley
eISSN:1574-6941
Copyright Holders:© 2008 Federation of European Microbiological Societies
Additional Information:Pubblicato online 11 gennaio 2008.
Deposited On:18 Aug 2009 10:05

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